CDw75 Antibody

• Description:

  Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This mAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• UniProt:

  P15907

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes were used as the immunogen for the CDw75 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgM k

• Clone:

  LN1

• Applications:

  FACS, IF, IHC-P

• Purity:

  PEG precipitation

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CDw75 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CDw75 antibody is available for research use only.

• Storage Conditions:

  Store the CDw75 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CDw75 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

• CAS Number:

  9007-83-4

• Location:

  Cell surface and cytoplasmic

• Image Legend:

  IHC analysis of formalin-fixed, paraffin-embedded normal human spleen stained with CDw75 antibody (clone LN1) .