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S100A9 Antibody

CAT: 0800-V2847-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2847-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP14) ; expressed by granulocytes, monocytes and by tissue macrophages. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This mAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cells (in inflamed tissues) .
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE; not suitable for frozen tissues) : 1-2 µg/mL for 30 min at RT
UniProt
P06702
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human protein was used as the immunogen for the S100A9 antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
S100A9/1011
Applications
IHC-P
Purity
PEG precipitation
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the S100A9 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This S100A9 antibody is available for research use only.
Storage Conditions
Store the S100A9 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the S100A9 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Location
Cytoplasmic
Image Legend
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with S100A9 antibody (clone S100A9/1011) .

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