Cytochrome C Antibody

CAT: 0800-V2787IHC-7MLSize: 7 mLDry Ice: NoHazardous: No

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CAT#:0800-V2787IHC-7MLSize:7 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Cytochrome C is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32) . Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached) .

CAS Number

9007-83-4

Specifications

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

UniProt

P99999

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 66-104 of pigeon Cytochrome C were used as the immunogen for the Cytochrome C antibody. It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

SPM389

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

Reconstitution

Store the Cytochrome C antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Cytochrome C antibody is available for research use only.

Storage Conditions

Store the Cytochrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

Location

Cytoplasmic

Image Legend

IHC: Formalin-fixed, paraffin-embedded human pancreas stained with Cytochrome C antibody (clone SPM389) .

UniProtKB · P99999

Cytochrome c

CYC_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P99999
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: CYCS
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: A4D166, B2R4I1, P00001, Q6NUR2, Q6NX69, Q96BV4

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Biological process

ApoptosisElectron transportRespiratory chainTransport

Disease

Disease variant

Ligand

HemeIronMetal-binding

Cellular component

Mitochondrion

Product Datasheet

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