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Cytochrome C Antibody

CAT: 0800-V2785-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V2785-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32) . Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL
UniProt
P99999
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Rat full-length protein was used as the immunogen for the Cytochrome C antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
6H2.B4
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Cytochrome C antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Cytochrome C antibody is available for research use only.
Storage Conditions
Store the Cytochrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Cytochrome C antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Flow cytometry testing of PFA fixed human HeLa cells with Cytochrome C antibody (clone 6H2.B4) ; Red=isotype control, Blue= Cytochrome C antibody.

UniProtKB · P99999

Cytochrome c

CYC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P99999
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CYCS
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A4D166, B2R4I1, P00001, Q6NUR2, Q6NX69, Q96BV4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Biological process

ApoptosisElectron transportRespiratory chainTransport

Disease

Disease variant

Ligand

HemeIronMetal-binding

Cellular component

Mitochondrion

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