PCNA Antibody

• Description :

  Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.

• CAS Number :

  9007-83-4

• Specifications :

  Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

• UniProt :

  P12004

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant full length human protein was used as the immunogen for the PCNA antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  PCNA/694

• Applications :

  FACS, WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This PCNA antibody is available for research use only.

• Storage Conditions :

  Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Location :

  Predominantly nuclear, some cytoplasmic

• Image Legend :

  Flow cytometry testing of PFA-fixed human HeLa cells with PCNA antibody (clone PCNA/694) ; Red=isotype control, Blue= PCNA antibody.