PCNA Antibody

• Description:

  Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

• UniProt:

  P12004

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full length human protein was used as the immunogen for the PCNA antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  PCNA/694

• Applications:

  FACS, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PCNA antibody is available for research use only.

• Storage Conditions:

  Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Predominantly nuclear, some cytoplasmic

• Image Legend:

  Flow cytometry testing of PFA-fixed human HeLa cells with PCNA antibody (clone PCNA/694) ; Red=isotype control, Blue= PCNA antibody.