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PAX7 Antibody

CAT: 0800-V2773-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2773-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma) .
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
UniProt
P23759
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human PAX7 protein corresponding to amino acids 301-505 was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
PAX7/1187
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the PAX7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PAX7 antibody is available for research use only.
Storage Conditions
Store the PAX7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the PAX7 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Nuclear
Image Legend
IHC testing of formalin-fixed, paraffin-embedded human Rhabdomyosarcoma with PAX7 antibody (clone PAX7/1187) . Required HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min.

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