Products for Research Use Only
RegisterLogin

NuMA Antibody

CAT: 0800-V2768-100UGSize: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-V2768-100UGSize:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Recognizes a phosphorylated protein of 228kDa, identified as nuclear mitotic apparatus protein (NuMA) . NuMA is intra-nuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 has been detected in patients with acute promyelocytic leukemia.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q14980
Host
Mouse
Reactivity
Human
Immunogen
Colon carcinoma 174T cells were used as the immunogen for the NuMA antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
SPM300
Applications
IHC-P
Purity
PEG precipitation
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the NuMA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NuMA antibody is available for research use only.
Storage Conditions
Store the NuMA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the NuMA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Location
Nuclear
Image Legend
IHC analysis of formalin-fixed, paraffin-embedded human tonsil stained with NuMA antibody (clone SPM300) .

Related Products

CatalogName
F41458-0.4MLNUMB Antibody
R35011-100UGNUMB Antibody
F41458-0.08MLNUMB Antibody
R31714NUMB Antibody
RQ8514NUMB Antibody
RQ8647NUMBL Antibody / NUMB-like
RQ8284NUMA1 Antibody / Nuclear mitotic apparatus protein 1
RQ4624NONO Antibody / p54nrb Antibody
F53954-0.2MLAntibody
F53954-0.05MLAntibody

Alternative Products

CatalogName
MBS9611179-01NuMA Antibody
JOT-AP06231-01NuMA Polyclonal Antibody
MBS9240343-01Anti-NUMA Antibody
C21995-20uLNuMA Antibody [K18C4]
326536-01NuMA

Popular Products