MFG-E8 Antibody

• Description:

  Recognizes a protein of 40-45kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  Q08431

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A human milk fat globule membrane preparation was used as the immunogen for the MFG-E8 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SPM291

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the MFG-E8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This MFG-E8 antibody is available for research use only.

• Storage Conditions:

  Store the MFG-E8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9007-83-4

• Location:

  Cell surface & cytoplasmic

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human breast carcinoma stained with MFG-E8 antibody (SPM291)