C-Kit Antibody

• Description:

  This mAb recognizes a protein of 145kDa, identified as CD117/p145kit/c-Kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117/c-Kit has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi's sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117/c-Kit is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.

• Specifications:

  Western blot: 0.5-1 µg/mL for 2 hours at RT

• UniProt:

  P10721

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant human fragment around amino acids 100-300 was used as the immunogen for the c-Kit antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  KIT/982

• Applications:

  WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the c-Kit antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This c-Kit antibody is available for research use only.

• Storage Conditions:

  Store the c-Kit antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the c-Kit antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface and cytoplasmic

• Image Legend:

  Western blot of a recombinant protein fragment with c-Kit antibody (KIT/982) .