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IDH1 Antibody

CAT: 0800-V2609IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V2609IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a 45kDa protein, which is identified as isocitrate dehydrogenase (IDH1) . It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming a-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of a-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
O75874
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment (119 amino acid residues around aa 280-420) from the human protein was used as the immunogen for the IDH1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
IDH1/1152
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the IDH1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This IDH1 antibody is available for research use only.
Storage Conditions
Store the IDH1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cytoplasmic & nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with IDH1 antibody (clone IDH1/1152) .

UniProtKB · O75874

Isocitrate dehydrogenase [NADP] cytoplasmic

IDHC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O75874
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IDH1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IDH, IDH1
EC number
1.1.1.42
Processing
—
Secondary accessions
Q567U4, Q6FHQ6, Q7Z3V0, Q93090, Q9NTJ9, Q9UKW8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Cellular component

CytoplasmPeroxisome

Biological process

Glyoxylate bypassTricarboxylic acid cycle

Ligand

MagnesiumManganeseMetal-bindingNADP

Molecular function

Oxidoreductase

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