GFAP Antibody

• Description:

  This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

• UniProt:

  P14136

• Host:

  Mouse

• Reactivity:

  Human, Rat

• Immunogen:

  Full length recombinant protein was used as the immunogen for the GFAP antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  ASTRO/789

• Applications:

  WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the GFAP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This GFAP antibody is available for research use only.

• Storage Conditions:

  Store the GFAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human cerebellum stained with GFAP antibody (ASTRO/789) .