Placental Alkaline Phosphatase Antibody

• Description:

  Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

• Specifications:

  Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL; 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2), Western blot: 1-2 µg/mL

• UniProt:

  P05187

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human ALPP protein was used as the immunogen for the Placental Alkaline Phosphatase antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  ALP/870

• Applications:

  IHC-P, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the Placental Alkaline Phosphatase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Placental Alkaline Phosphatase antibody is available for research use only.

• Storage Conditions:

  Store the Placental Alkaline Phosphatase antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic and cell surface

• Image Legend:

  Western blot testing of human JEG-3 cells with Placental Alkaline Phosphatase antibody (clone ALP/870) . Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.