TRIM29 Antibody

• Description:

  It recognizes a 66kDa protein, which is identified as Tripartite motif-containing protein 29 (TRIM29) . It interacts with the intermediate filament protein vimentin, a substrate for the PKC family of protein kinases, and with hPKCI-1, an inhibitor of the PKCs. TRIM29 protein contains both zinc finger and leucine zipper motifs, suggesting that the it may form homodimers and possibly associate with DNA. High expression of TRIM29 has been reported in gastric cancer and pancreatic cancer, and correlates with enhanced tumor growth and lymph node metastasis. TRIM29 is also able to distinguish lung squamous cell carcinoma from lung adenocarcinoma with ~90% positive accuracy, when used in a panel with TTF-1, p63, CK5/6, and Napsin-A antibodies.

• Specifications:

  ELISA (order BSA/sodium azide-free format for coating), Western blot: 1-2 µg/mL

• UniProt:

  Q14134

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment (126 amino acid residues between aa 1-200) from the human protein was used as the immunogen for the TRIM29 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  TRIM29/1042

• Applications:

  ELISA, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the TRIM29 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This TRIM29 antibody is available for research use only.

• Storage Conditions:

  Store the TRIM29 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the TRIM29 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic & cell surface

• Image Legend:

  Western blot testing of a 1) partial recombinant protein and 2) A431 cell lysate using TRIM29 antibody (clone TRIM29/1042) . Predicted molecular weight ~66 kDa.