CD35 Antibody

• Description:

  Recognizes a protein of 210-220kDa, which is identified as the complement receptor 1 (CR1) /CD35. This mAb does not block CR1 activity. It is highly specific to CR1 and shows no cross-reaction with CR2. The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This mAb labels follicular dendritic cells and follicular dendritic cell sarcoma.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• UniProt:

  P17927

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as the immunogen for the CD35 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CR1/802

• Applications:

  FACS, IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD35 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD35 antibody is available for research use only.

• Storage Conditions:

  Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human tonsil stained with CD35 antibody (CR1/802) .