TOP1MT Antibody

• Description :

  DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking and rejoining DNA strands. Due to their roles in DNA replication, recombination, and transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

• Specifications :

  Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1) (2)

• UniProt :

  Q969P6

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant full-length human protein was used as the immunogen for the TOP1MT antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  TOP1MT/488

• Applications :

  FACS, WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution :

  Store the TOP1MT antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This TOP1MT antibody is available for research use only.

• Storage Conditions :

  Store the TOP1MT antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic (mitochondria)

• Image Legend :

  Immunofluorescent staining of PFA-fixed human MCF7 cells with TOP1MT antibody (clone TOP1MT/488, green) and Reddot nuclear stain (red) .