Semaphorin-4D Antibody / SEMA4D / CD100

• Description:

  Recognizes a homodimeric protein comprised of 50kDa subunits, identified as Semaphorin-4D, or CD100. It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.

• Specifications:

  Functional studies (order BSA/sodium azide-free format), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

• UniProt:

  Q92854

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  PHA stimulated human peripheral blood lymphocytes were used as the immunogen for the Semaphorin-4D antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  A8

• Applications:

  Functional studies, FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the Semaphorin-4D antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Semaphorin-4D antibody is available for research use only.

• Storage Conditions:

  Store the Semaphorin-4D antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Semaphorin-4D antibody to be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  SDS-PAGE Analysis of Purified, BSA-Free Semaphorin-4D Antibody (clone A8) . Confirmation of Integrity and Purity of the Antibody.