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CEA Antibody / CEACAM5

CAT: 0800-V2445-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2445-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes proteins of 80-200kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This mAb reacts with nonspecific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+) . Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT (1) (2)
UniProt
P06731
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human protein was used as the immunogen for the CEA antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
C66/195
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CEA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CEA antibody is available for research use only.
Storage Conditions
Store the CEA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CEA antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min
Location
Cytoplasmic and luminal surface
Image Legend
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with CEA antibody (C66/195) .
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