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P27 Antibody

CAT: 0800-V2437-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2437-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb co-precipitates cdk4 in complex p27Kip1 and is excellent for staining of formalin-fixed tissues.
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT
UniProt
P46527
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Mouse recombinant protein was used as the immunogen for the p27 antibody. The epitope has been localized to amino acids 83-204.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
DCS-72.F6
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the p27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This p27 antibody is available for research use only.
Storage Conditions
Store the p27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC: Formalin-fixed, paraffin-embedded human cervical cancer stained with p27 antibody (DCS-72.F6)

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