CD19 Antibody

• Description:

  CD19 is expressed on follicular dendritic cells and B cells. It is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. [Wiki]

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  P15391

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human CD19 recombinant full length protein was used as immunogen for this CD19 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CVID3/429

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  CD19 antibody with azide can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

• Limitations:

  This CD19 antibody is available for research use only.

• Storage Conditions:

  CD19 antibody with azide can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Variations in protocols, secondaries and substrates may require the CD19 antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  Immunofluorescent staining of human Raji cells with CD19 antibody (clone CVID3/429, green) and Reddot nuclear stain (red) .