BrdU Antibody

CAT: 0800-V2321SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V2321SAF-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 1-3 µg/mL
Host
Mouse
Reactivity
All Species Expected
Immunogen
Bromodeoxyuridine conjugated to KLH was used as the immunogen.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
BRD469
Applications
IHC-P, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This BrdU antibody is available for research use only.
Storage Conditions
Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC.

Location
Nuclear
Image Legend
IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD469) .
Product Datasheet
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