BrdU Antibody

• Description :

  It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to Bromodeoxyuridine is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Immunofluorescence: 1-3 µg/mL

• Host :

  Mouse

• Reactivity :

  All Species Expected

• Immunogen :

  Bromodeoxyuridine conjugated to KLH was used as the immunogen.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  BRD469

• Applications :

  IHC-P, IF

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the BrdU antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This BrdU antibody is available for research use only.

• Storage Conditions :

  Store the BrdU antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC.
  

• CAS Number :

  9007-83-4

• Location :

  Nuclear

• Image Legend :

  IHC staining of mouse intestine tissue (10X) with BrdU antibody (BRD469) .