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Biotin Antibody

CAT: 0800-V2319SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2319SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
Host
Mouse
Immunogen
Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
Hyb-8
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Biotin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Biotin antibody is available for research use only.
Storage Conditions
Store the Biotin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Location
Depends on target localization
Image Legend
IHC staining of human tonsil with biotinylated lambda light chain antibody followed by anti-Biotin antibody (clone Hyb-8) . Note cell membrane & cytoplasmic staining.
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