PCNA Antibody

• Description :

  This antibody recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA) . PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.

• Specifications :

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

• Host :

  Mouse

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  PC10

• Applications :

  FACS, IF, WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This PCNA antibody is available for research use only.

• Storage Conditions :

  Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number :

  9007-83-4

• Location :

  Predominantly nuclear, some cytoplasmic

• Image Legend :

  Western blot testing of HeLa cell lysate with PCNA antibody (clone PC10) . Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.