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PCNA Antibody

CAT: 0800-V2218-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V2218-100UGSize:100 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA) . PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
PC10
Applications
FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PCNA antibody is available for research use only.
Storage Conditions
Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Predominantly nuclear, some cytoplasmic
Image Legend
Western blot testing of HeLa cell lysate with PCNA antibody (clone PC10) . Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.

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