PCNA Antibody

• Description:

  This antibody recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA) . PCNA is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA-cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This antibody is excellent for the detection of PCNA in multiple applications.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  PC10

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PCNA antibody is available for research use only.

• Storage Conditions:

  Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Predominantly nuclear, some cytoplasmic

• Image Legend:

  Western blot testing of HeLa cell lysate with PCNA antibody (clone PC10) . Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.