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PAX7 Antibody

CAT: 0800-V2217-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V2217-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The PAX gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the PAX family of proteins have been linked to disease and cancer in humans. PAX7 is a protein specifically expressed in cultured satellite cell- derived myoblasts. In situ hybridization reveals that PAX7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding PAX7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma) .
CAS Number
9007-83-4
Specifications
Immunofluorescence: 0.5-1 µg/mL, Flow cytometry: 0.5-1ug/10^6 cells, Western blot: 0.5-1 µg/mL
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human PAX7 protein corresponding to amino acids 301-505 was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
PAX7/497
Applications
IF, FACS, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the PAX7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PAX7 antibody is available for research use only.
Storage Conditions
Store the PAX7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Location
Nuclear
Image Legend
Western blot testing of human HeLa cell lysate with PAX7 antibody (clone PAX7/497) . Expected molecular weight: 55-57 kDa.

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