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Myogenin Antibody

CAT: 0800-V2206-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V2206-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6) . MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Myogenin antibody labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms’ tumor.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P15173
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Human myogenin recombinant protein was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MGN185
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Myogenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Myogenin antibody is available for research use only.
Storage Conditions
Store the Myogenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC testing of FFPE rhabdomyosarcoma stained with Myogenin antibody (clone MGN185) .

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