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EpCAM Antibody

CAT: 0800-V2184BTNSize: 500 µLDry Ice: NoHazardous: No
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CAT#:0800-V2184BTNSize:500 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
CAS Number
9007-83-4
Specifications
Flow cytometry: 5ul per test per one million cells or 5ul per 100ul of whole blood, Immunofluorescence: 2-4 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL at RT
UniProt
P16422
Host
Mouse
Reactivity
Human
Immunogen
Small cell lung carcinoma cells were used as the immunogen for the EpCAM antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
VU-1D9
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Biotin Conjugate
Buffer
0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the EpCAM antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).
Limitations
This EpCAM antibody is available for research use only.
Storage Conditions
Store the EpCAM antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .
Formulation
0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the EpCAM antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
IHC staining of FFPE human colon carcinoma with biotinylated EpCAM antibody (clone VU-1D9) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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