Lambda Light Chain Antibody

• Description:

  This antibody is specific to lambda light chain of immunoglobulin and shows no cross-reaction with kappa light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. In general the ratio of Kappa to Lambda is 3:1. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.

• Specifications:

  ELISA: 1-5 µg/mL for coating (order BSA/sodium azide-free format)

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Purified human Ig was used as the immunogen for this Lambda Light Chain antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  ICO-106

• Applications:

  ELISA

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the Lambda Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Lambda Light Chain antibody is available for research use only.

• Storage Conditions:

  Store the Lambda Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Lambda Light Chain antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic and secreted

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free Lambda Light Chain antibody (clone ICO-106) as confirmation of integrity and purity.