CD50 Antibody

CAT: 0800-V2138-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V2138-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes the D1 domain of an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM3 (Leucocyte Workshop VI) . It is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This CD50 antibody is excellent for staining of formalin/paraffin tissues.
CAS Number
9007-83-4
Specifications
Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (order BSA/sodium azide-free format), Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for this CD50 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
186-2G9
Applications
Blocking, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the CD50 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD50 antibody is available for research use only.
Storage Conditions
Store the CD50 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Membrane, cytoplasm
Image Legend
IHC analysis of FFPE human tonsil with CD50 antibody (clone 186-2G9) .

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