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HIF1 alpha Antibody

CAT: 0800-V2132SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2132SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (alpha and beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIFs regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human HIF1 alpha was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
HIF1A-84
Applications
FACS, IF
Purity
Protein G purified antibody
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the HIF1 alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This HIF1 alpha antibody is available for research use only.
Storage Conditions
Store the HIF1 alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HIF1 alpha antibody to be titered up or down for optimal performance.
Location
Nuclear, possible cytoplasmic
Image Legend
Immunofluorescent staining of methanol-fixed human HeLa cells with HIF1 alpha antibody (green) and Reddot nuclear stain (red) .

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