HIF1 alpha Antibody

• Description:

  HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (alpha and beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIFs regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human HIF1 alpha was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  HIF1A-84

• Applications:

  FACS, IF

• Purity:

  Protein G purified antibody

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the HIF1 alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This HIF1 alpha antibody is available for research use only.

• Storage Conditions:

  Store the HIF1 alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HIF1 alpha antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Nuclear, possible cytoplasmic

• Image Legend:

  Immunofluorescent staining of methanol-fixed human HeLa cells with HIF1 alpha antibody (green) and Reddot nuclear stain (red) .