GFAP Antibody

• Description:

  This antibody recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein. The mAb shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. It is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. GFAP antibody is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

• Specifications:

  Flow cytometry: 1-2ug/million, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  GFAP isolated from pig spinal cord was used as the immunogen.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  GA-5

• Applications:

  FACS, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the GFAP antibody at 2-8oC.

• Limitations:

  This GFAP antibody is available for research use only.

• Storage Conditions:

  Store the GFAP antibody at 2-8°C.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC staining of FFPE human cerebellum with GFAP antibody (clone GA-5) .