Products for Research Use Only
RegisterLogin

MART-1 Antibody

CAT: 0800-V2116-20UGSize: 20 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-V2116-20UGSize:20 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. There are seven other melanoma associated antigens recognized by autologous cytotoxic T cells: MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MART-1 antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. The antibody does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
CAS Number
9007-83-4
Specifications
Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human MART-1 protein was used as the immunogen for this antibody.
Clonality
Monoclonal
Isotype
IgG2b λ
Clone
M2-7C10
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MART-1 antibody is available for research use only.
Storage Conditions
Store the MART-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MART-1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human melanoma stained with MART-1 antibody (clone M2-7C10) . Note cytoplasmic staining of cells. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.

Related Products

CatalogName
V5289SAF-100UGMART-1 / Melan-A Antibody
V5289-20UGMART-1 / Melan-A Antibody
V5290SAF-100UGMelan-A Antibody / MART-1
V5290-100UGMelan-A Antibody / MART-1
V5290-20UGMelan-A Antibody / MART-1
V5289-100UGMART-1 / Melan-A Antibody
V8659SAF-100UGRecombinant Melan-A Antibody / MART-1
V8659-100UGRecombinant Melan-A Antibody / MART-1
V8659-20UGRecombinant Melan-A Antibody / MART-1
V5603-20UGRecombinant MLANA Antibody / Melan-A / MART-1

Alternative Products

CatalogName
GTR18188820-01MART-1 Antibody
20R-1858MART 1 antibody

Popular Products