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EGFR Antibody

CAT: 0800-V2105-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2105-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head/neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
The immunizing protein for this antibody was recombinant human EGFR.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
GFR450
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This EGFR antibody is available for research use only.
Storage Conditions
Store the EGFR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Location
Cell surface
Image Legend
FACS testing of A431 cells with isotype control (gray), without primary antibody (black) and EGFR antibody (green, clone GFR450) .

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