CD35 Antibody

• Description:

  This antibody recognizes a protein of 210-220 kDa, which is identified as the complement receptor 1 (CR1), or CD35. The antibody is specific for a site on CD35 that is distal from the C3b-binding site, so that it is unable to block CD35 activity. The CD35 antibody is highly specific and shows no cross-reaction with CR2. The primary function of CD35 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. The Knops blood group system is a system of antigens located on this protein. Follicular dendritic cells (FDC) are restricted to the B-cell regions of secondary lymphoid follicles. They are CD21+/CD35+/CD1a-. This antibody labels follicular dendritic cells and follicular dendritic cell sarcoma.

• Specifications:

  Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Intact human monocytes were used as the immunogen for this CD35 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  E11

• Applications:

  FACS, IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the CD35 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD35 antibody is available for research use only.

• Storage Conditions:

  Store the CD35 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  IHC testing of human tonsil stained with CD35 antibody (E11) . Note cell membrane staining.