CD33 Antibody

• Description:

  Recognizes a 67kDa glycoprotein, which is identified as CD33 (HLDA IV; WS Code M-505) . CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM) -containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.

• Specifications:

  Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood

• UniProt:

  P20138

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human AML cells were used as the immunogen for this CD33 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  WM53

• Applications:

  FACS

• Purity:

  Protein G affinity chromatography

• Format:

  CF488 Conjugate

• Buffer:

  500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD33 antibody at 2-8oC, protected from light.

• Limitations:

  This CD33 antibody is available for research use only.

• Storage Conditions:

  Store the CD33 antibody at 2-8°C, protected from light.

• Formulation:

  500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD33 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface