CD28 Antibody

• Description:

  This antibody recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical Tcell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing Tcells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. it is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Functional studies (order BSA/sodium azide-free format)

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as the immunogen for this CD28 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CB28

• Applications:

  FACS, IF, Functional studies

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1X PBS, pH 7.4

• Reconstitution:

  Store the CD28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD28 antibody is available for research use only.

• Storage Conditions:

  Store the CD28 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD28 antibody to be titered up or down for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  Flow cytometry staining of PFA-fixed human Jukat cells with CD28 antibody (clone CB28) ; Red=isotype control, Blue= CD28 antibody.