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CD5 Antibody

CAT: 0800-V2038-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V2038-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a 67kDa transmembrane protein, which is identified as CD5. It is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in Tcell areas. CD5 antibody is a pan Tcell marker that also reacts with a range of neoplastic Bcells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large Bcell lymphoma. Aberrant expression is useful in making a diagnosis of mature Tcell neoplasms. CD5 antibody detection is diagnostic in CLL/SLL within a panel of other Bcell markers, especially one that includes anti-CD23. CD5 antibody is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, it can be used in distinguishing thymic carcinoma (+) from thymoma (-) . It does not react with granulocytes or monocytes.
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Functional studies (order BSA/sodium azide-free format), Immunoprecipitation (0.5-1ug/500ug protein lysate)
Host
Mouse
Reactivity
Human
Immunogen
Stimulated human leukocytes were used as the immunogen for this CD5 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
CRIS-1
Applications
FACS, IF, Functional studies, IP
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the CD5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD5 antibody is available for research use only.
Storage Conditions
Store the CD5 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD5 antibody to be titered up or down for optimal performance.
Location
Cell surface
Image Legend
FACS staining of human PBM cells using CD5 antibody (clone CRIS-1) .

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