Rbfox3 Antibody / Fox3 / Neun

• Description:

  RNA binding protein, fox-1 homolog (C. elegans) 3 (Rbfox3) is a protein that in humans is encoded by the RBFOX3 gene. This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q8BIF2

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant mouse protein (amino acids H28-D304) was used as the immunogen for the Rbfox3 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, IF, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Rbfox3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Rbfox3 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Rbfox3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Rbfox3 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus, Cytoplasm

• Image Legend:

  IHC staining of FFPE mouse brain tissue with Rbfox3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.