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CIP4 Antibody / TRIP10

CAT: 0800-RQ6812Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6812Size:100 µg
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Description
Cdc42-interacting protein 4 is a protein that in humans is encoded by the TRIP10 gene. TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2) . Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q15642
Host
Rabbit
Reactivity
Human, Rat
Immunogen
Recombinant human protein (amino acids M1-M182) was used as the immunogen for the CIP4 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the CIP4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This CIP4 antibody is available for research use only.
Storage Conditions
After reconstitution, the CIP4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the CIP4 antibody should be determined by the researcher.
Location
Cytoplasmic, cell membrane
Image Legend
Immunofluorescent staining of FFPE human A431 cells with CIP4 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · Q15642

Cdc42-interacting protein 4

CIP4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q15642
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TRIP10
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R8A6, B7WP22, D6W645, O15184, Q53G22, Q5TZN1, Q6FI24, Q8NFL1, Q8TCY1, Q8TDX3, Q96RJ1
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneCell projectionCytoplasmCytoskeletonGolgi apparatusLysosomeMembrane

Domain

Coiled coilSH3 domain

Biological process

Endocytosis

Ligand

Lipid-binding

PTM

Phosphoprotein

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