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HSD3B1/2 Antibody

CAT: 0800-RQ6736Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6736Size:100 µg
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Description
HSD3B1 is a human gene that encodes for a 3beta-hydroxysteroid dehydrogenase/delta (5) -delta (4) isomerase type I or hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1. It is mapped to 1p12. The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL
UniProt
P14060
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids Q17-Q373) was used as the immunogen for the HSD3B1/2 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
3F3C/7F9C8
Applications
WB, IF
Purity
Affinity purified
Format
Purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the HSD3B1/2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This HSD3B1/2 antibody is available for research use only.
Storage Conditions
After reconstitution, the HSD3B1/2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the HSD3B1/2 antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Immunofluorescent staining of FFPE human U-2 OS cells with HSD3B1/2 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · P14060

3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

3BHS1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P14060
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HSD3B1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K691, Q14545, Q8IV65
Protein keywords

Technical term

Direct protein sequencingProteomics identificationReference proteome

Cellular component

Endoplasmic reticulumMembraneMitochondrion

Molecular function

IsomeraseMultifunctional enzymeOxidoreductase

Biological process

Lipid metabolismSteroid metabolismSteroidogenesis

Ligand

NADNADP

Domain

TransmembraneTransmembrane helix

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