TRMT1 Antibody

CAT: 0800-RQ6689Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ6689Size:100 µg
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Description
This gene encodes a tRNA-modifying enzyme that acts as a dimethyltransferase, modifying a single guanine residue at position 26 of the tRNA. The encoded enzyme has both mono- and dimethylase activity when exogenously expressed, and uses S-adenosyl methionine as a methyl donor. The C-terminal region of the encoded protein has both a zinc finger motif, and an arginine/proline-rich region. Mutations in this gene have been implicated in autosomal recessive intellectual disorder (ARID) . Alternative splicing results in multiple transcript variants encoding different isoforms. There is a pseudogene of this gene on the X chromosome.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9NXH9
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human protein (amino acids A61-P626) was used as the immunogen for the TRMT1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the TRMT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This TRMT1 antibody is available for research use only.
Storage Conditions
After reconstitution, the TRMT1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the TRMT1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human HeLa cells with TRMT1 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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