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Prolyl Endopeptidase Antibody / PREP

CAT: 0800-RQ6509Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6509Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in humans is encoded by the PREP gene. The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P48147
Host
Rabbit
Reactivity
Human, Rat
Immunogen
An E. coli-derived human protein (amino acids M1-E227) was used as the immunogen for the Prolyl Endopeptidase antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Prolyl Endopeptidase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Prolyl Endopeptidase antibody is available for research use only.
Storage Conditions
After reconstitution, the Prolyl Endopeptidase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Prolyl Endopeptidase antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC staining of FFPE human skin cancer tissue with Prolyl Endopeptidase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

UniProtKB · P48147

Prolyl endopeptidase

PPCE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P48147
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PREP
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PE
EC number
3.4.21.26
Processing
—
Secondary accessions
Q8N6D4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Acetylation

Cellular component

Cytoplasm

Molecular function

HydrolaseProteaseSerine protease

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