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ORC5L Antibody

CAT: 0800-RQ6504Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6504Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Origin recognition complex subunit 5 is a protein that in humans is encoded by the ORC5 (ORC5L) gene. The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Alternatively spliced transcript variants encoding distinct isoforms have been described.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
O43913
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
Amino acids HDHPPEYSADFYAAYINI from the human protein were used as the immunogen for the ORC5L antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the ORC5L antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ORC5L antibody is available for research use only.
Storage Conditions
After reconstitution, the ORC5L antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the ORC5L antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human MCF-7 cells with ORC5L antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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