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NDE1 Antibody / NudE

CAT: 0800-RQ6499Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6499Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nuclear distribution protein nudE homolog 1 is a protein that in humans is encoded by the NDE1 gene. This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization, mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4, a disorder characterized by lissencephaly, severe brain atrophy, microcephaly, and severe cognitive disability. Alternative splicing results in multiple transcript variants.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
Q9NXR1
Host
Rabbit
Reactivity
Human
Immunogen
An E. coli-derived human protein (amino acids E74-C335) was used as the immunogen for the NDE1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the NDE1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This NDE1 antibody is available for research use only.
Storage Conditions
After reconstitution, the NDE1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the NDE1 antibody should be determined by the researcher.
Image Legend
IHC staining of FFPE human breast cancer tissue with NDE1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Product Datasheet
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