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Ki67 Antibody

CAT: 0800-RQ6498Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-RQ6498Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
E9PVX6
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An E. coli-derived mouse protein (amino acids F29-S3177) was used as the immunogen for the Ki67 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IHC-P, Direct ELISA
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the Ki67 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Ki67 antibody is available for research use only.
Storage Conditions
After reconstitution, the Ki67 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Ki67 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE mouse lymph tissue with Ki67 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Product Datasheet
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