Chromogranin A Antibody

• Description:

  CHGA, also known as CGA or chromogranin A, is an acidic protein costored and coreleased with catecholamines from storage granules in the adrenal medulla. CHGA is a member of the granin family of neuroendocrine secretory protein, It is located in secretory vesicles of neurons and endocrine cells. In humans, CHGA protein is encoded by the CHGA gene, and it is mapped to 14q32.12. CHGA gene product is a precursor to three biologically active peptides: vasostatin, pancreastatin, and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine) .

• Specifications:

  Western blot: 1-2 µg/mL, Flow cytometry: 1-3ug/million cells, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  P26339

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  An E. coli-derived mouse protein (amino acids D378-K425) was used as the immunogen for the Chromogranin A antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, FACS, IHC-P, Direct ELISA

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Chromogranin A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Chromogranin A antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Chromogranin A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Chromogranin A antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry testing of mouse HEPA1-6 cells with Chromogranin A antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= Chromogranin A antibody.