Integrin linked protein kinase Antibody / ILK

• Description:

  ILK, also known as Integrin-linked kinase, is a serine-threonine protein kinase. Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK) interacts with the cytoplasmic domain of beta-1 integrin. This gene was initially described to encode a serine/ threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. Recent results showed that ILK contains 5 ankyrin-like repeats, and that the C-terminal kinase domain is actually a pseudo-kinase with adaptor function.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

• UniProt:

  Q13418

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant human protein (amino acids R9-K452) was used at the immunogen for the Integrin linked protein kinase antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IF, IHC-P, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the Integrin linked protein kinase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Integrin linked protein kinase antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Integrin linked protein kinase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the Integrin linked protein kinase antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  IHC staining of FFPE bladder epithelial carcinoma tissue with Integrin linked protein kinase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.