GRB10 Antibody

• Description:

  GRB10, Growth factor receptor-bound protein 10, also known as insulin receptor-binding protein Grb-IR is a protein that in humans is encoded by the GRB10 gene. The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors (e.g., IGF1R and IGF2R) . Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner. Alternatively spliced transcript variants encoding different isoforms have been identified.

• Specifications:

  Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  Q13322

• Host:

  Mouse

• Reactivity:

  Human, Monkey, Rat

• Immunogen:

  Recombinant human protein (amino acids M1-K251) was used as the immunogen for the GRB10 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  5H7

• Applications:

  WB, FACS

• Purity:

  Antigen affinity purified

• Format:

  Purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the GRB10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This GRB10 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the GRB10 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the GRB10 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry testing of human ThP-1 cells with GRB10 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= GRB10 antibody.