U2AF2 Antibody / U2AF65

CAT: 0800-RQ6283Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ6283Size:100 µg
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Description
Splicing factor U2AF 65 kDa subunit is a protein that in humans is encoded by the U2AF2 gene. It is mapped to 19q13.42. U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
P26368
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A human recombinant partial protein (amino acids M238-H470) was used as the immunogen for the U2AF2 antibody.
Clonality
Monoclonal
Isotype
IgG2a
Clone
3G9
Applications
WB, IHC-P, IF, FACS
Purity
Affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
After reconstitution, the U2AF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This U2AF2 antibody is available for research use only.
Storage Conditions
After reconstitution, the U2AF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the U2AF2 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of FFPE human A549 cells with U2AF2 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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